Additionally, selective index (SI) showed that MUSC 137 extract was less toxic against normal cell lines when compared to MCF-7 and HCT-116 cells. The results indicated MCF-7 cells were most susceptible to the extract with the lowest IC 50 (61.33 ± 17.10 μg/mL), followed by HCT-116 and A549. The results revealed MUSC 137 possesses significant antioxidant activity and demonstrates cytotoxic effect against several cancer cell lines tested. Antioxidant activity was examined via DPPH assay while the cytotoxic effect was assessed by means of examining the activity of the extract against selected human cancer cell lines, namely colon cancer cells (HCT-116, Caco-2, SW480, and HT-29), breast cancer cell (MCF-7), lung cancer cell (A549), prostate cancer cell (DU145), and cervical cancer cell (Ca Ski). In order to examine the bioactivities possessed by the strain, fermentation extract was prepared through solvent extraction method prior to bioactivities screenings. Uniquely adapted microorganisms from mangrove habitats have previously yielded compounds of biopharmaceutical interest. We investigated the phylogenetic, genomic, biochemical, and phenotypic characteristics of this strain. Streptomyces pluripotens MUSC 137 was isolated from mangrove soil obtained from Tanjung Lumpur, Pahang, Malaysia. 3Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
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2UKM Medical Molecular Biology Institute–UKM Medical Centre, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.1Biomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway, Malaysia.Hooi-Leng Ser 1, Nurul-Syakima Ab Mutalib 2, Wai-Fong Yin 3, Kok-Gan Chan 3, Bey-Hing Goh 1* and Learn-Han Lee 1*